Journal: International Journal of Molecular Sciences

Article Title: Seizures in PPT1 Knock-In Mice Are Associated with Inflammatory Activation of Microglia

doi: 10.3390/ijms23105586

Figure Lengend Snippet: Changes in inflammatory cytokines IL-1β and TNF-α in PPT1 KI mice. ( A ) The expression level of IL-1β in the hippocampus from PPT1 KI mice and WT mice. ( B ) TNF-α protein in hippocampal tissue measured by ELISA assay. * p < 0.05.

Article Snippet: The protein concentrations of IL-1β and TNF-α were quantified using an ELISA kit (4A Biotech Co., Ltd., Beijing, China) according to the manufacturer’s instruction for 7-month-old PPT1 KI mice and age-matched WT mice.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Clinical and Translational Gastroenterology

Article Title: Apolipoprotein C3 Is Downregulated in Patients With Inflammatory Bowel Disease

doi: 10.14309/ctg.0000000000000500

Figure Lengend Snippet: Multivariable analysis of the differences in lipid profile and apolipoprotein C3 between patients with IBD and controls

Article Snippet: An ELISA kit was used for the detection of ApoC3 (Elabscience Biotechnology, China).

Techniques:

Journal: Frontiers in Immunology

Article Title: A Telomerase-Derived Peptide Exerts an Anti-Hepatitis B Virus Effect via Mitochondrial DNA Stress-Dependent Type I Interferon Production

doi: 10.3389/fimmu.2020.00652

Figure Lengend Snippet: The anti-HBV effect of GV1001 on human hepatocytes. (A) HBsAg and extracellular HBV DNA levels were evaluated. PBS (0.5%), GV1001 (10 μM), and LMV (10 μM) were added to HepG2 cells and Huh-7 cells transiently transfected with the pHBV-1.2X-wild-type plasmid. (B) Cell viability assays (MTS) were conducted. (C) Quantitative PCR was performed on the supernatant of HepG2 cells transfected with 1.2x-WT plasmid and treated with GV1001 at different doses. The IC50 was calculated. (D,E) Extracellular HBV virion and HBeAg levels were measured using qPCR and ELISAs from the supernatants of HepG2-2.15 cells after the administration of phosphate-buffered saline and GV1001 for 48 h. (F) A lactate dehydrogenase assay was carried out on HepG2-2.15 and HepG2 cells to determine the cytotoxicity induced by GV1001. Data represent the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 versus PBS.

Article Snippet: For the detection of 8-hydroxy-2′-deoxyguanosine (8-OHdG) activity, competitive ELISAs from an 8-OHdG analysis kit (OxiSelect Oxidative DNA Damage ELISA kit, Cell Biolabs, San Diego, CA, United States) was used according to the manufacturer’s protocol.

Techniques: Transfection, Plasmid Preparation, Real-time Polymerase Chain Reaction, Saline, Lactate Dehydrogenase Assay

Journal: Frontiers in Immunology

Article Title: A Telomerase-Derived Peptide Exerts an Anti-Hepatitis B Virus Effect via Mitochondrial DNA Stress-Dependent Type I Interferon Production

doi: 10.3389/fimmu.2020.00652

Figure Lengend Snippet: Anti-HBV effect of GV1001 on a transgenic mouse model. (A) Female transgenic mice were injected with phosphate-buffered saline, lamivudine, (0.5 mg/kg) or GV1001 (0.05 mg/kg), twice weekly. At 4 and at 8 weeks later, HBsAg from mouse serum was measured by ELISAs. (B) After the extraction of viral DNA from serum, HBV DNA was quantified by qPCR. This study was conducted in accordance with the guidelines established by the Seoul National University Institutional Animal Care and Use Committee (Approval No. SNU-111025-6-3). Data represent the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 versus PBS.

Article Snippet: For the detection of 8-hydroxy-2′-deoxyguanosine (8-OHdG) activity, competitive ELISAs from an 8-OHdG analysis kit (OxiSelect Oxidative DNA Damage ELISA kit, Cell Biolabs, San Diego, CA, United States) was used according to the manufacturer’s protocol.

Techniques: Transgenic Assay, Injection, Saline, Extraction